Mauricio Pochettino confirmed fan fears on Tuesday but put no time frame on the England frontman’s return.
However, reports claim the problem could see Kane on the sidelines for up to two months.
It means the 23-year-old could miss a number of crucial Premier League and Champions League clashes, including the north London derby on November 6.
Kane sustained the injury in Sunday’s 1-0 victory over Sunderland, shortly after he scored the only goal in the White Hart Lane clash.
He was stretchered off the pitch after an awkward fall from a tackle, and he later left the stadium on crutches with a protective boot on his right foot.
The club were waiting the results of tests on Monday to fully determine the damage, and while they didn’t reveal the full extent of the problem, they did at least confirm there was no break.
Reports claim the striker has suffered grade two ligament damage, which would normally require six to eight weeks of rehabilitation.
“He got a ligament ankle problem and we need to assess every day and see,” Pochettino said.
“Today it’s impossible to give a time for a recovery. I am not a doctor. I can only explain what the doctors said to me.
“He is in a very good mood. We chatted before in the restaurant, he’s in a very good way.
“There are different rumours, we cannot guess whether it’s eight weeks, 10, four, one. It’s impossible to assess today and know how long.”
Injuries are never nice but part of the game – working hard to get fit as soon as possible! Will be cheering on the lads while I recover.pic.twitter.com/0BZ3Ih03ts
— Harry Kane (@HKane) September 20, 2016
Kane has endured an exhausting two years after he played all of Tottenham’s 38 Premier League games last season, in between consecutive summers representing England, first at under-21 level and then at Euro 2016.
Pochettino admitted the only positive was that his star man will now have some time to recuperate.
“We must take that. We need to be positive now the damage is done,” Pochettino said.
“It’s a good opportunity to build his body, his fitness, his mentality and to work hard on different aspects and help him become more strong.”
Kane’s absence also hands an opportunity to summer signing Vincent Janssen, the Dutch striker brought in from AZ Alkmaar in a deal worth £18.5million in July.
Janssen scored 27 goals for AZ last season but is yet to open his account for his new club.
“Vincent arrives in a good moment – he’s ready to compete,” Pochettino said.
“You can see his qualities. It’s always difficult when you don’t have an opportunity to play – strikers are always excited to play and sometimes feel the pressure.
“Now he needs to be calm and he has an opportunity tomorrow to compete.”
Read more at http://talksport.com/football/tottenham-blow-spurs-confirm-harry-kane-ankle-ligament-injury-put-no-time-frame-return#QH6WBGaIXCj85Bh3.99