Benfica, who opened the scoring after just two minutes, led 16-0 at half-time, and finished the match with nine different goalscorers.
The side was formed at the end of 2017 and has several international players in their squad.
However, as a new team, they have to compete in the second division.
Darlene de Souza – who joined the club in April – scored eight of the side’s goals.
The result is the largest margin of victory in senior football in Portugal – the previous highest was Sporting’s 21-0 win in the Portuguese Cup in 1971.
The club’s directors congratulated the players on their attitude, adding: “together we are stronger”.